High resolution microscopy.

Biophysical Imaging

Prof. Dr. Christian EGGELING
High resolution microscopy.
Image: Private
Prof. Christian Eggeling Prof. Christian Eggeling Image: Jan-Peter Kasper (University of Jena)

Prof. Dr. Christian EGGELING

Email: christian.eggeling@uni-jena.de
Phone: +49 3641-9-47670

In 2012, Professor Eggeling started as a principal investigator in the MRC Human Immunology Unit and as the scientific director of the newly established Wolfson Imaging Centre Oxford at the Weatherall Institute of Molecular Medicine, University of Oxford, and was appointed Professor of Molecular Immunology in 2014, positions which he still holds today. In December 2017, he started as a Professor of Superresolution Microscopy and director of the Institute of Applied Optics and Biophysics (IAOB) at the Friedrich Schiller University Jena, and as the Head of the Department of Biophysical Imaging at the Leibniz IPHT Jena.

Research Areas

The research group of Christian Eggeling is focused on the development of advanced microscopy for the investigation of molecular organization and dynamics in cells, especially on the cellular plasma membrane. Highlights are the optimization of superresolution STED microscopy and its combination with single-molecule fluorescence spectroscopy tools such as fluorescence correlation spectroscopy (FCS), use of adaptive optics for deep-tissue investigations, advancements in singleparticle tracking (using fluorescence, interferometric scattering (iSCAT) and novel superresolution MINFLUX microscopy), the detailed investigation of lipid membrane heterogeneity such as lipid rafts, and biological applications of all of these tools for investigations of multiple biomedical issues such as within the Excellence Cluster “Balance of the Microverse”, the Collaborative Research Center 1278 PolyTarget, infection diagnostics or immunology. Further, fully serviced user microscope facilities have been set up and are being optimized. 

Teaching Fields

Main teaching activities include bachelor biophysics lectures and exercises, master applied laser technology lectures and exercises as well as support of physics teaching practical. Further, HiWis, master and bachelor as well as PhD students are welcome and supported through various research projects.

Research Methods

The Eggeling group is specialized on advanced fluorescence microscopy techniques, especially superresolution STED microscopy in combination with fluorescence correlation spectroscopy (STED-FCS), and has access to multiple microscopes including confocal, wide-field/TIRF superresolution, structured illumination and STED microscopes, but is also using complementary approaches such as single-particle tracking and intererometric Scattering (iSCAT) microscopy. Further, a novel superresolution MINFLUX microscope was installed. Otherwise, access to biochemical wet labs, cell culture and optical labs in the Zentrum für Angewandte Forschung and the Leibniz Institute of Photonic Technology is granted (biosafety 1 and 2 space).

Recent Research Results

. . Picture: Eggeling research group.

Recent research includes the use of artificial intelligence algorithms for an optimized fluorescence microscopy/ spectroscopy analysis [1], advanced microscopy of molecular interactions involved in virus infection [2-4], use of adaptive optics for optimized inner-cellular and tissue observations [5, 6], and the biophysical characterization of immune responses [7, 8].

[1] Waithe et al., J. Cell Biol. 219, e201903166 (2020).
[2] Favard et al., Science Adv. 5, eaaw8651 (2019).
[3] Chojnacki et al., Nature Commun. 8, 545 (2017).
[4] Carravilla et al., Nature Commun. 10, 78 (2019).
[5] Barbotin et al., Opt. Express 27, 23378 (2019).
[6] Barbotin et al., ACS Photonics 7, 1742 (2020).
[7] Fritzsche et al., Sci. Adv. 3, e1603032 (2017).
[8] Santos et al., Nature Immunol. 19, 203 (2019).

link to the Eggeling research group