The research group of Christian Eggeling is focused on the development of advanced microscopy for the investigation of molecular organization and dynamics in cells, especially on the cellular plasma membrane.Christian Eggeling’s research focus. Highlights are the optimization of superresolution STED microscopy and its combination with single-molecule fluorescence spectroscopy tools such as fluorescence correlation spectroscopy (FCS), use of adaptive optics for deep-tissue investigations, advancements in single-particle tracking (using fluorescence, interferometric scattering (iSCAT) and novel superresolution Minflux microscopy), the detailed investigation of lipid membrane heterogeneity such as lipid rafts, and biological applications of all of these tools for investigations of multiple biomedical issues such as within the Excellence Cluster “Balance of the Microverse” (studying the influence of the microbiome, https://microverse-cluster.de/), the Collaborative Research Center 1278 Polytarget (use of nanoparticles for drug delivery, www.polytarget.uni-jena.de), infection diagnostics (Leibniz Institute of Photonic Technology, www.leibniz-ipht.de) or immunology (Weatherall Institute of Molecular Medicine Oxford, www.imm.ox.ac.uk). Further, fully serviced user microscope facilities have been set up and are being optimized (Microverse Imaging Center Jena, Wolfson Imaging Center Oxford). For further details see www.biophysical-imaging.com.
Main teaching activities include bachelor biophysics lectures and exercises, applied laser technology lectures and exercises as well as support of physics teaching practical. Further, HiWis, master and bachelor as well as PhD students are welcome and supported through various research projects.
The Eggeling group is specialized on advanced fluorescence microscopy techniques, especially superresolution STED microscopy in combination with fluorescence correlation spectroscopy (STED-FCS), and has access to multiple microscopes including confocal, wide-field/TIRF superresolution, structured illumination and STED microscopes, but is also using complementary approaches such as single-particle tracking and intererometric Scattering (iSCAT) microscopy. Further, a novel superresolution MINFLUX microscope was installed. Otherwise, access to biochemical wet labs, cell culture and optical labs in the Zentrum für Angewandte Forschung and the Leibniz Institute of Photonic Technology is granted (biosafety 1 and 2 space).