Microscopy sample inspection.
Watermark

ACP scientist Heintzmann publishes in Nature Methods

Together with colleagues from several Universities and Institutes in the USA, ACP principal scientist Rainer Heintzmann has most recently published an original research article titled "Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution" in Nature Methods (Chakraborty et al., Nature Methods 16, 1109, 2019). Congratulations!
Microscopy sample inspection.
Image: Jan-Peter Kasper (University of Jena)
  • Light

Published: 6 November 2019, 09:51 | By: Christian Helgert

Together with colleagues from several Universities and Institutes in the USA, ACP principal scientist Rainer Heintzmann has most recently published an original research article titled "Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution" in Nature Methods (Chakraborty et al., Nature Methods 16, 1109, 2019). Congratulations!

Abstract: We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell-cell interactions.

link to the full article

link to ACP's publication highlights

Share this page
The Friedrich Schiller University on social media:
Studying amid excellence:
  • Logo of the "Total E-Quality" initiative
  • Logo of the best practice club "Family in Higher Education Institutions"
  • Logo of the "Partner University of High Performance Sports" project
  • Logo of quality of German Accreditation Council - system accredited
Top of the page