INSTITUTE OF APPLIED PHYSICS - ACP scientist Heintzmann publishes in Nature Methods

ACP scientist Heintzmann publishes in Nature Methods

Together with colleagues from several Universities and Institutes in the USA, ACP principal scientist Rainer Heintzmann has most recently published an original research article titled "Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution" in Nature Methods (Chakraborty et al., Nature Methods 16, 1109, 2019). Congratulations!

Abstract: We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell-cell interactions.

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News from: 06.11.2019
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